Two new eudesmane sesquiterpene glucosides from the aerial parts of Artemisia vulgaris

Abstract

Using combined chromatographic methods, two new sesquiterpene glucosides, vulgarosides A ( 1 ) and B ( 2 ), and two known analogs ainsliaside E ( 3 ) and pumilaside A ( 4 ) were isolated from the aerial parts of Artemisia vulgaris . Their chemical structures were established by spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1 D and 2 D-NMR) spectroscopy and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS). In addition, their cytotoxicity on five human cancer cell lines, including KB (epidermoid carcinoma), HepG2 (hepatocarcinoma), MCF7 (breast carcinoma), SK-Mel-2 (melanoma), and LNCaP (prostate cancer) was also evaluated by the SRB assay. However, none of the tested eudesmane sesquiterpene glycosides showed significant cytotoxicity (IC 50 >100 µM).